Overall, yeast performed the best at improving CHO culture growth and productivity without being detrimental to downstream protein A processes but could affect mAb product glycan distribution while Ex-Cell CD yielded lower titers but has less effect on glycosylation. Ex-Cell CD performed the best at maintaining glycan profile compared to the non-supplemented cultures.
#Ivcd cell culture software
Integral viable cell concentration (IVCD), understood as the concentration of viable cells that were working during the time the cell culture lasted, was calcu-lated as area under curve by trapezium rule using GraphPad Prism Software v5. Profiling of the glycan types revealed that yeast and soybean hydrolysates could affect the distribution of galactosylated glycans. mula Qp P/IVCD, where P is the antibody concentration at the end of culture 11. t2 and t1 (h) and IVCD (cell h mL1 ) is the integral viable cell density. Adding these three hydrolysates neither increased the amount of host cell protein, DNA or aggregate impurity amounts nor affect their clearance after purification. decreased integrated viable cell density (IVCD), increased cell death, and higher specic productivity with increased glucose concentration. Optimizing amino acid composition of CHO cell culture media for a fusion. IgG recovery from protein A purification was not compromised for all cultures by adding yeast, soybean and Ex-Cell CD hydrolysates except for one sample from soybean supplemented culture. Wheat hydrolysates increased peak viable cell density but did not improve productivity. Yeast, soybean and Ex-Cell CD improved the final mAb titer by increasing the specific productivity (qP) and/or extension of the culture period. We supplemented two commercial Chinese hamster ovary (CHO) culture media with hydrolysates from four different sources, yeast, soybean, Ex-Cell CD (a chemically defined hydrolysate replacement) and wheat to improve the productivity of two cell lines expressing different monoclonal antibodies (mAbs). Two semisteady states with a viable cell density of 5. Plant and yeast derived hydrolysates are economical and efficient alternative medium supplements to improve mammalian cell culture performance. Semisteady state cultures are useful for studying cell physiology and facilitating media development.